Cytoplasmic domains of Ca²⁺-ATPase gather upon phosphoenzyme isomerization as if producing axis of action (blue cylinder) in which conformational energy gained by Actuator (scarlet)-Phosphorylation (yellow) domain interactions at two pink-highlighted regions is transmitted to cause possibly further inclination and twisting of transmembrane helices for releasing bound Ca²⁺.  Depicted on decavanadate-bound model (PDB accession code 1FQU), the analogue of Ca²⁺-released phosphoenzyme.